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001 978-1-60327-019-9
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020 _a9781603270199
_9978-1-60327-019-9
024 7 _a10.1007/978-1-60327-019-9
_2doi
050 4 _aTP248.13-.65
072 7 _aTCB
_2bicssc
072 7 _aTEC009010
_2bisacsh
072 7 _aTCB
_2thema
082 0 4 _a660.63
_223
245 1 0 _aTransgenesis Techniques
_h[electronic resource] :
_bPrinciples and Protocols /
_cedited by Elizabeth J. Cartwright.
250 _a3rd ed. 2009.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2009.
300 _aXII, 335 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v561
505 0 _aTransgenesis in Various Model Systems -- Transgenesis in Drosophila melanogaster -- Transgenesis in Caenorhabditis elegans -- Transgenesis in Zebrafish with the Tol2 Transposon System -- Generation of Transgenic Frogs -- Pronuclear DNA Injection for the Production of Transgenic Rats -- Transgenesis in the Mouse -- Cell-Type-Specific Transgenesis in the Mouse -- Transgene Design and Delivery into the Mouse Genome: Keys to Success -- Overexpression Transgenesis in Mouse: Pronuclear Injection -- Gene-Targeting Vectors -- Gene Trap: Knockout on the Fast Lane -- Culture of Murine Embryonic Stem Cells -- Targeting Embryonic Stem Cells -- Generation of Chimeras by Microinjection -- Generation of Chimeras by Morula Aggregation -- Surgical Techniques for the Generation of Mutant Mice -- Site-Specific Recombinases for Manipulation of the Mouse Genome -- Cre Transgenic Mouse Lines -- Large-Scale Mouse Mutagenesis -- Dedicated Mouse Production and Husbandry -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains -- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recovery and Distribution of Conserved Mutant Strains.
520 _aOne of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.
650 0 _aBiotechnology.
650 0 _aMedical genetics.
650 0 _aGenetics.
650 0 _aBiology
_xTechnique.
650 1 4 _aChemical Bioengineering.
650 2 4 _aMedical Genetics.
650 2 4 _aGenetics and Genomics.
650 2 4 _aExperimental Organisms.
700 1 _aCartwright, Elizabeth J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781603270182
776 0 8 _iPrinted edition:
_z9781607611134
776 0 8 _iPrinted edition:
_z9781627038416
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v561
856 4 0 _uhttps://doi.org/10.1007/978-1-60327-019-9
912 _aZDB-2-PRO
999 _c7837
_d7837