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001 978-1-60761-974-1
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008 101116s2011 xxu| s |||| 0|eng d
020 _a9781607619741
_9978-1-60761-974-1
024 7 _a10.1007/978-1-60761-974-1
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a576.5
_223
245 1 0 _aTransgenic Mouse Methods and Protocols
_h[electronic resource] /
_cedited by Marten H. Hofker, Jan van Deursen.
250 _a2nd ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXI, 352 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v693
505 0 _aIntroduction: Strategies for developing genetically modified mice -- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection -- Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos -- Cryopreservation of mouse spermatozoa and In vitro fertilization -- Autopsy and histological analysis of the transgenic mouse -- Transgene design -- Inducible Transgenic Mouse Models -- Lentiviral transgenesis -- Transgenesis in Mouse Embryonic Stem Cells -- Engineering BAC Reporter Gene Constructs for Mouse Transgenesis -- Targeting Vector Construction Through Recombineering -- Generating Conditional Knockout Mice -- Hypomorphic mice -- MICER Targeting Vectors for Manipulating the Mouse Genome -- Knockin approaches -- Generation of a series of knockin alleles using RMCE in ES cells -- Selection of targeted mutants from a library of randomly mutagenized ES cells -- Generation of genetically modified rodents using random ENU mutagenesis -- Bone marrow transplantations to study gene function in hematopoietic cells -- Adenovirus Mediated Gene Transfer.
520 _aThe generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
650 0 _aGenetics.
650 0 _aBiotechnology.
650 0 _aMedical genetics.
650 1 4 _aGenetics and Genomics.
650 2 4 _aChemical Bioengineering.
650 2 4 _aMedical Genetics.
700 1 _aHofker, Marten H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _avan Deursen, Jan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607619734
776 0 8 _iPrinted edition:
_z9781607619758
776 0 8 _iPrinted edition:
_z9781493956760
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v693
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-974-1
912 _aZDB-2-PRO
999 _c7774
_d7774