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020 _a9781627031462
_9978-1-62703-146-2
024 7 _a10.1007/978-1-62703-146-2
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI049000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572.6
_223
245 1 0 _aMass Spectrometry of Glycoproteins
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Jennifer J. Kohler, Steven M. Patrie.
250 _a1st ed. 2013.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2013.
300 _aXI, 339 p. 90 illus., 12 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v951
520 _a Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.
650 0 _aProteins .
650 0 _aMass spectroscopy.
650 1 4 _aProtein Biochemistry.
650 2 4 _aMass Spectrometry.
700 1 _aKohler, Jennifer J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPatrie, Steven M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627031455
776 0 8 _iPrinted edition:
_z9781627031479
776 0 8 _iPrinted edition:
_z9781493962037
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v951
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-146-2
912 _aZDB-2-PRO
999 _c7766
_d7766