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003 DE-He213
005 20260610083708.0
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008 100301s2004 xxu| s |||| 0|eng d
020 _a9781592596652
_9978-1-59259-665-2
024 7 _a10.1385/1592596657
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a576.5
_223
245 1 0 _aDrosophila Cytogenetics Protocols
_h[electronic resource] /
_cedited by Daryl S. Henderson.
250 _a1st ed. 2004.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2004.
300 _aXIII, 468 p. 269 illus., 30 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v247
505 0 _aThe Chromosomes of Drosophila melanogaster -- Spermatogenesis -- Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy -- Immunocytological Analysis of Oogenesis -- Cytological Analysis of Oogenesis -- Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants -- Feulgen-DNA Cytophotometry for Estimating C Values -- Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary -- Studying Nuclear Organization in Embryos Using Antibody Tools -- Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos -- Orcein Staining and the Identification of Polytene Chromosomes -- Salivary Chromosome Analysis of Aberrations -- In Situ Hybridization to Polytene Chromosomes -- Combined Immunostaining and FISH Analysis of Polytene Chromosomes -- Electron Microscopy of Polytene Chromosomes -- Analysis of Mitosis in Squash Preparations of Larval Brains -- Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo -- Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling -- Immunostaining of Squash Preparations of Chromosomes of Larval Brains -- Visualizing Mitosis in Whole-Mount Larval Brains -- Immunostaining of Whole-Mount Imaginal Discs -- Wing Somatic Mutation and Recombination Test -- Analysis of Histoblasts -- Visualizing Apoptosis -- RNAi in Cultured Drosophila Cells.
520 _aProbably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators.
650 0 _aGenetics.
650 1 4 _aGenetics and Genomics.
700 1 _aHenderson, Daryl S.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588290502
776 0 8 _iPrinted edition:
_z9781617373251
776 0 8 _iPrinted edition:
_z9781489928153
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v247
856 4 0 _uhttps://doi.org/10.1385/1592596657
912 _aZDB-2-PRO
999 _c7736
_d7736