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007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592596782
_9978-1-59259-678-2
024 7 _a10.1385/1592596789
_2doi
050 4 _aQH491-492
072 7 _aPSC
_2bicssc
072 7 _aSCI072000
_2bisacsh
072 7 _aPSC
_2thema
082 0 4 _a571.8
_223
245 1 0 _aMolecular Methods in Developmental Biology
_h[electronic resource] :
_bXenopus and Zebrafish /
_cedited by Matt Guille.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aXII, 217 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v127
505 0 _aThe Animal Cap Assay -- Cell and Tissue Transplantation in Zebrafish Embryos -- Ribonuclease Protection Analysis of Gene Expression in Xenopus -- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) -- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos -- In Situ Hybridization to Sections of Xenopus Embryos -- Zebrafish Immunohistochemistry -- Immunohistochemistry of Xenopus Embryos -- Preparation and Testing of Synthetic mRNA for Microinjection -- Microinjection into Xenopus Oocytes and Embryos -- Microinjection into Zebrafish Embryos -- Expression from DNA Injected into Xenopus Embryos -- Promoter Analysis in Zebrafish Embryos -- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs -- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis -- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis -- Mapping Protein-DNA Interactions Using In Vivo Footprinting.
520 _aIn Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.
650 0 _aDevelopmental biology.
650 1 4 _aDevelopmental Biology and Stem Cells.
700 1 _aGuille, Matt.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896037908
776 0 8 _iPrinted edition:
_z9781617371899
776 0 8 _iPrinted edition:
_z9781489943316
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v127
856 4 0 _uhttps://doi.org/10.1385/1592596789
912 _aZDB-2-PRO
999 _c7734
_d7734