000 03722nam a22004695i 4500
001 978-1-59259-687-4
003 DE-He213
005 20260610083708.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592596874
_9978-1-59259-687-4
024 7 _a10.1385/1592596878
_2doi
050 4 _aRC254-282
072 7 _aMJCL
_2bicssc
072 7 _aMED062000
_2bisacsh
072 7 _aMJCL
_2thema
082 0 4 _a616.994
_223
245 1 0 _aCytotoxic Drug Resistance Mechanisms
_h[electronic resource] /
_cedited by Robert Brown, Uta Böger-Brown.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aXI, 238 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v28
505 0 _aDrug Resistance -- Cell Sensitivity Assays -- Cell Sensitivity Assays -- Cell Sensitivity Assays -- Analysis of Apoptosis in Tissue Sections -- Measurement of P-glycoprotein Function -- Measuring MDR-1 by Quantitative RT-PCR -- Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method -- Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography -- Topoisomerase I and II Activity Assays -- 5-Fluorouracil Metabolizing Enzymes -- Measuring DNA Adducts by Immunoassay (ELISA) -- Measuring Drug-DNA Adducts in Individual Cells -- Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single-Cell Gel Electrophoresis (Comet) Assay -- PCR Analysis of Microsatellite Instability -- O 6-Alkylguanine-DNA Alkyltransferase Assay -- Analysis of the p53 Status of Tumors -- Bcl-2 Family Immunohistochemistry -- Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization -- Genetic Analysis of Drug Resistance by Reverse In Situ Hybridization.
520 _aIn Cytotoxic Drug Resistance Mechanisms, leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms makes it possible to test established and laboratory-derived hypotheses with clinically derived tumor samples. State-of-the-art and readily reproducible, the methods presented here afford basic and clinical scientists a powerful complement of tools for investigating all the clinically relevant mechanisms used as markers of the biological response to anticancer chemotherapeutics today.
650 0 _aOncology.
650 1 4 _aOncology.
700 1 _aBrown, Robert.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBöger-Brown, Uta.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896036031
776 0 8 _iPrinted edition:
_z9781617370939
776 0 8 _iPrinted edition:
_z9781489941725
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v28
856 4 0 _uhttps://doi.org/10.1385/1592596878
912 _aZDB-2-PRO
999 _c7723
_d7723