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001 978-1-59259-183-1
003 DE-He213
005 20260610083708.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592591831
_9978-1-59259-183-1
024 7 _a10.1385/1592591833
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aCalcium-Binding Protein Protocols
_h[electronic resource] :
_bVolume 1: Reviews and Case Studies /
_cedited by Hans J. Vogel.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXVI, 340 p. 133 illus., 4 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v172
505 0 _aand Reviews -- Calcium-Binding Proteins -- Calcium -- Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation -- The Multifunctional S100 Protein Family -- Ca2+Binding to Proteins Containing ?-Carboxyglutamic Acid Residues -- The Caseins of Milk as Calcium-Binding Proteins -- Calcium-Binding Proteins: Case Studies -- Preparation of Recombinant Plant Calmodulin Isoforms -- Isolation of Recombinant CardiacTroponin C -- Skeletal Muscle Troponin C -- Purification of Recombinant Calbindin D9k -- S100 Proteins -- Cadherins -- ?-Lactalbumin and (Calcium-Binding) Lysozyme -- Recombinant Annexin II Tetramer -- Purification and Characterization of ALG-2 A Novel Apoptosis-Linked Ca2+-Binding Protein -- Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain -- Neurocalcin -- Crystallization and Structure-Function of Calsequestrin -- Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains -- Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin -- Study of Calcineurin Structure by Limited Proteolysis.
520 _aCalcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols-Volume 1: Reviews and Case Studies and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. This first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second companion volume, Methods and Techniques, focuses on cutting-edge experimental methods for studying solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aVogel, Hans J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896036888
776 0 8 _iPrinted edition:
_z9781617371356
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v172
856 4 0 _uhttps://doi.org/10.1385/1592591833
912 _aZDB-2-PRO
999 _c7720
_d7720