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020 _a9781627039680
_9978-1-62703-968-0
024 7 _a10.1007/978-1-62703-968-0
_2doi
050 4 _aRB155-155.8
072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
_2thema
082 0 4 _a616.042
_223
245 1 0 _aHoming Endonucleases
_h[electronic resource] :
_bMethods and Protocols /
_cedited by David R. Edgell.
250 _a1st ed. 2014.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2014.
300 _aX, 284 p. 60 illus., 39 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1123
505 0 _aHoming Endonucleases:  From Genetic Anomalies to Programmable Genomic Clippers -- Bioinformatic Identification of Homing Endonucleases and Their Target Sites -- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes -- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein -- Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE -- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis -- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases -- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection -- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile -- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling -- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries -- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display -- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes -- Bioinformatics Identification of Coevolving Residues -- Identification and Analysis of Genomic Homing Endonuclease Target Sites -- Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
520 _aHoming Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.
650 0 _aMedical genetics.
650 0 _aBiotechnology.
650 1 4 _aMedical Genetics.
650 2 4 _aChemical Bioengineering.
700 1 _aEdgell, David R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627039673
776 0 8 _iPrinted edition:
_z9781627039697
776 0 8 _iPrinted edition:
_z9781493963195
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1123
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-968-0
912 _aZDB-2-PRO
999 _c7717
_d7717