000 05495nam a22004695i 4500
001 978-1-59259-568-6
003 DE-He213
005 20260610083708.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592595686
_9978-1-59259-568-6
024 7 _a10.1385/0896034887
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aTransmembrane Signaling Protocols
_h[electronic resource] /
_cedited by Dafna Bar-Sagi.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXIII, 328 p. 47 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v84
505 0 _aOverviews -- Peptide Recognition Mechanisms of Eukaryotic Signaling Modules -- Protein–Protein Interactions in Signaling Cascades -- Transmembrane Signaling by Receptor Oligomerization -- Specific Topics -- Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases -- Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries -- Selective Antagonism of Receptor Signaling Using Antisense RNA to Deplete G-Protein Subunits -- Microinjection of Antisense Oligonucleotides and Electrophysiological Recording of Whole-Cell Currents as Tools to identify Specific G-Protein Subtypes Coupling Hormone Receptors to Voltage-Gated Calcium Channels -- Oocytes Microinjection Assay to Study the MAP-Kinase Cascade -- Mammalian Cell Microinjection Assay to Study the Function of Rac and Rho -- Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS -- Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade -- Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade -- Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D -- Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D -- Two-Hybrid Analysis of Ras—Raf Interactions -- Two-Hybrid Analysis of Ras–Raf Interactions -- Cloning and Mutational Analysis of the She-Phosphotyrosine Interaction/ Phosphotyrosine-Binding Domain -- Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domain -- Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins -- Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins -- Prenylation Assays for SmallGTPases -- Prenylation Assays for Small GTPases -- Analysis of Myristoylated and Palmitoylated Src Family Proteins -- Analysis of Myristoylated and Palmitoylated Src Family Proteins -- Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded Phospholipid Vesicles -- Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded Phospholipid Vesicles -- Biochemical and Biological Analyses of Farnesyl-Protein Transferase Inhibitors -- Biochemical and Biological Analyses of Farnesyl-Protein Transferase Inhibitors -- Identification and Characterization of Small GTPase-Associated Kinases -- Identification and Characterization of Small GTPase-Associated Kinases -- Functional Studies of Dual-Specificity Phosphatases -- Functional Studies of Dual-Specificity Phosphatases.
520 _aTransmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aBar-Sagi, Dafna.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896034327
776 0 8 _iPrinted edition:
_z9780896034884
776 0 8 _iPrinted edition:
_z9781489942548
776 0 8 _iPrinted edition:
_z9781489942555
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v84
856 4 0 _uhttps://doi.org/10.1385/0896034887
912 _aZDB-2-PRO
999 _c7689
_d7689