| 000 | 05559nam a22004695i 4500 | ||
|---|---|---|---|
| 001 | 978-1-59259-270-8 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083707.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s1999 xxu| s |||| 0|eng d | ||
| 020 |
_a9781592592708 _9978-1-59259-270-8 |
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| 024 | 7 |
_a10.1385/1592592708 _2doi |
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| 050 | 4 | _aQH573-671 | |
| 072 | 7 |
_aPSF _2bicssc |
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| 072 | 7 |
_aSCI017000 _2bisacsh |
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| 072 | 7 |
_aPSF _2thema |
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| 082 | 0 | 4 |
_a571.6 _223 |
| 245 | 1 | 0 |
_aMolecular Embryology _h[electronic resource] / _cedited by Paul T. Sharpe, Ivor Mason. |
| 250 | _a1st ed. 1999. | ||
| 264 | 1 |
_aTotowa, NJ : _bHumana Press : _bImprint: Humana, _c1999. |
|
| 300 |
_aXVI, 756 p. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v97 |
|
| 505 | 0 | _aThe Mouse Embryo -- The Mouse as a Developmental Model -- Culture of Postimplantation Mouse Embryos -- Organ Culture in the Analysis of Tissue Interactions -- Treatment of Mice with Retinoids In Vivo and In Vitro -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Gene Trapping in Mouse Embryonic Stem Cells -- Production and Growth of Conditionally Immortal Cell Lines from the H-2Kb tsA58 Transgenic Mouse -- Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Mouse Primordial Germ Cells -- Chicken Embryo -- The Avian Embryo -- Chick Embryos -- New Culture -- Grafting Hensen’s Node -- Grafting of Somites -- Notochord Grafts -- Transplantation of Avian Neural Tissue -- Grafting of Apical Ridge and Polarizing Region -- Tissue Recombinations in Collagen Gels -- Quail-Chick Chimeras -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Amphibian Embryo -- An Overview of Xenopus Development -- Mesoderm Induction Assays -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- A Method for Generating Transgenic Frog Embryos -- Axolotl/newt -- Zebrafish -- The Zebrafish -- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development -- Transgenic Zebrafish -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function -- Retinoids in Nonmammalian Embryos -- Monvertevrate Chordates -- Protochordates -- Retroviruses -- Gene Transfer to the Rodent Embryo by Retroviral Vectors.-Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Differential Display of Eukaryotic mRNA -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Single-Cell RT-PCR cDNA Subtraction -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- In Situ Hybridization to RNA in Whole Embryos -- Wholemount In Situ Hybridization to Xenopus Embryos -- Wholemount In Situ Hybridization to Amphioxus Embryos -- In Situ Hybridization to Sections (Nonradioactive) -- Immunohistochemistry Using Polyester Wax -- Immunohistochemistry on Whole Embryos -- Whole Embryo Assays for Programmed Cell Death -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos -- Protein Techniques -- Microscopy and Photography -- Microscopy and Photomicrography Techniques. | |
| 520 | _aIn Molecular Embryology, expert investigators provide a comprehensive guide to the cutting-edge methods used today across the dramatically growing field of vertebrate molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The major techniques of experimental molecular biology and the particular advantages of each different species are emphasized. Detailed, easy-to-follow protocols, together with relevant background information and helpful tips, optimize the methods for success. Molecular Embryology brings together in one volume all the major techniques and common experimental species needed to study the mechanisms of biological development in vertebrates. Bound to become a standard reference in this field, the book makes it possible for experienced and novice researchers alike to move between embryos of diverse vertebrate classes as their project progresses, ensuring their ability to utilize the experimental advantages of different systems to address specific developmental questions. | ||
| 650 | 0 | _aCytology. | |
| 650 | 1 | 4 | _aCell Biology. |
| 700 | 1 |
_aSharpe, Paul T. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aMason, Ivor. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896033870 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489943262 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489943279 |
| 830 | 0 |
_aMethods in Molecular Biology, _x1940-6029 ; _v97 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1385/1592592708 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c7644 _d7644 |
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