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003 DE-He213
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007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592592609
_9978-1-59259-260-9
024 7 _a10.1385/0896035212
_2doi
050 4 _aRC1-1245
072 7 _aMJC
_2bicssc
072 7 _aMED022000
_2bisacsh
072 7 _aMJC
_2thema
082 0 4 _a616
_223
245 1 0 _aHepatitis C Protocols
_h[electronic resource] /
_cedited by Johnson Y. N. Lau.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXXI, 621 p. 171 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v19
505 0 _aDetection of Anti-HCV in Serum -- Detection of Anti-GOR Antibodies in HCV Infection -- Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III -- Detection and Quantitation of HCV in Serum -- Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization -- Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA -- Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology -- Quality Control of the Polymerase Chain Reaction -- Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays -- Detection of HCV in Liver Tissue -- Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR -- Quantification of HCV RNA in Liver Tissue by bDNA Assay -- HCV Genotypes -- Hepatitis C Virus -- Molecular Evolutionary Analysis -- Genotyping by Type-Specific Primers That Can Type HCV Types 1–6 -- Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region -- Determination of HCV Genotypes by RFLP -- HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II -- Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region -- Determination of HCV Quasispecies by Cloning and Sequencing -- Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism -- Hepatitis C Virus Heteroduplex Tracking Assay -- Detection of HCV In Situ -- In Situ Detection of HCV -- In Situ Detection of Hepatitis C Viral Antigens -- In Situ Hybridization and the Detection and Localization of HCV RNA -- The In Situ Detection of PCR-Amplified Hepatitis C RNA -- lmmunoelectron Microscopic Characterization ofHCV -- Molecular Biologic Characterization -- Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones -- Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing -- Use of a Discistronic Vector for the Quantitation of HCV IRES Activity -- Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli -- Expression and Characterization of the HCV NS2 Protease -- Expression and Characterization of HCV NS3 Protease -- Expression and Characterization of the HCV NS3 Helicase Domain -- Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase) -- Detection and Molecular Cloning of the Extreme 3?-End of HCV -- In Vitro Stability of Hepatitis C Virus RNA -- Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome -- HCV-Specific Immunologic Response -- Role of Immune Response in HCV -- Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC -- Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay -- Determination of HCV-Specific Bulk CD8+ Activity in Liver -- Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue -- Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization -- In Vitro Culture Model -- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification -- Strand-Specific rTth RT-PCR for the Analysis of HCV Replication -- Cell Culture Systems for the Detection of HCV Infection -- Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines -- Primary Human Hepatocyte Culture for the Study of HCV -- A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication -- Construction ofRecombinant Viral Vectors for the Expression of HCV Genes -- Construction of Recombinant Vaccinia Virus Expressing HCV Genes -- Construction of Recombinant Adeno-Associated Virus (AAV) -- Production of Replication-Deficient Adenovirus Recombinants -- Generation of Recombinant Herpes Simplex Virus Ampiicons -- Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products -- In Vivo Models -- The Chimpanzee Model -- Liver Transplantation as a Model to Study Hepatitis C Virus Infection.
520 _aIn Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.
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532 8 _aInaccessible, or known limited accessibility
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650 0 _aDiseases.
650 1 4 _aDiseases.
700 1 _aLau, Johnson Y. N.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035218
776 0 8 _iPrinted edition:
_z9781489943477
776 0 8 _iPrinted edition:
_z9781489943484
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v19
856 4 0 _uhttps://doi.org/10.1385/0896035212
912 _aZDB-2-PRO
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