000 06551nam a22005055i 4500
001 978-1-59259-254-8
003 DE-He213
005 20260610083705.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592592548
_9978-1-59259-254-8
024 7 _a10.1385/0896034720
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aFree Radical and Antioxidant Protocols
_h[electronic resource] /
_cedited by Donald Armstrong.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXVII, 455 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v108
505 0 _aTechniques for the Measurement of Oxidative Stress -- Oxygen Consumption Methods -- Spin Trapping and Electron Paramagnetic Resonance Spectroscopy -- In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy -- Single Photon Counting -- Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence -- Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography -- Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid -- Aromatic Hydroxylation -- Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma -- Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma -- Antibodies Against Malondialdehyde-Modified Proteins -- Oxidized LDL and Lp(a) -- Oxidized and Unoxidized Fatty Acyl Esters -- Synthesis of Lipid and Cholesterol Hydroperoxide Standards -- Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography -- Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo -- Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC -- Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA -- Nitric Oxide Synthase -- Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR -- A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity -- Autofluorescent Ceroid/Lipofuscin -- In Vivo Technique for Autofluorescent Lipopigments -- An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism -- Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion -- Techniques for the Measurement of Antioxidant Activity -- Simultaneous Determination of Serum Retinol, Tocopherols, and Carotenoids by HPLC -- EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E -- Nonvitamin Plasma Antioxidants -- Regulatory Antioxidant Enzymes -- In Vitro Screening for Antioxidant Activity -- Antioxidant Activity of Low-Density Lipoprotein -- Lipoic Acid as an Antioxidant -- Reduced Glutathione and Glutathione Disulfide -- Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples -- Techniques Applicable to Both Oxidative Stress and Antioxidant Activity -- A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro -- Analysis of Oxidation and Antioxidants Using Microtiter Plates -- Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy -- Energy Dispersive X-Ray Microanalysis -- Pro-Oxidant and Antioxidant Effects of Estrogens -- Artificial Radical Generating and Scavenging Systems.
520 _aIn Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or descriptions for non-text content (e.g., images and graphs). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at through this accessibility request webform if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aArmstrong, Donald.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896034723
776 0 8 _iPrinted edition:
_z9781617370496
776 0 8 _iPrinted edition:
_z9781489942487
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v108
856 4 0 _uhttps://doi.org/10.1385/0896034720
912 _aZDB-2-PRO
999 _c7556
_d7556