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020 _a9781617792618
_9978-1-61779-261-8
024 7 _a10.1007/978-1-61779-261-8
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aSingle Molecule Enzymology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Gregory I. Mashanov, Christopher Batters.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXI, 294 p. 88 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v778
505 0 _aSingle Enzyme Studies: A Historical Perspective -- Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins -- Studying Kinesin’s Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer -- Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors -- Snapshots of Kinesin Motors on Microtubule Tracks -- Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy -- Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle -- Quantum Dot Labeling Strategies to Characterize Single Molecular Motors -- Imaging Individual Myosin Molecules Within Living Cells -- Single Molecule Measurements Using Microneedles -- Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology -- Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers -- A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases -- Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes -- Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers -- Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging -- Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor -- Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors.
520 _aThe last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy.  In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.
650 0 _aBiochemistry.
650 0 _aEnzymology.
650 1 4 _aBiochemistry.
650 2 4 _aEnzymology.
700 1 _aMashanov, Gregory I.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBatters, Christopher.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617792601
776 0 8 _iPrinted edition:
_z9781617792625
776 0 8 _iPrinted edition:
_z9781493961900
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v778
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-261-8
912 _aZDB-2-PRO
999 _c7505
_d7505