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003 DE-He213
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007 cr nn 008mamaa
008 140206s1998 xxu| s |||| 0|eng d
020 _a9781592592142
_9978-1-59259-214-2
024 7 _a10.1385/0896033546
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aFlow Cytometry Protocols
_h[electronic resource] /
_cedited by Mark J. Jaroszeski, Richard Heller.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aX, 274 p. 56 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v91
505 0 _aBasics of Flow Cytometry -- Detection of Terminal Transferase in Leukemia -- The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells -- Detection of Intracellular/lntranuclear Antigens -- Detection of Intracellular/lntranuclear Antigens in Tumor Cells -- Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry -- Estimation of Cell Viability by Flow Cytometry -- Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential -- Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH -- Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry -- Estimation of Drug Resistance by Flow Cytometry -- Flow Cytometric Monitoring of Cellular Pharmacokinetics -- Quantification of Xenoprotein Electroinsertion in Mammalian Cells -- Flow Cytometry Quantification of Electropermeabilization -- Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products -- Cell Cycle Analysis of Asynchronous Populations -- Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies -- Solid Tumor DNA Ploidy Analysis -- FISHing for Cytokines -- Analysis of Apoptosis by Flow Cytometry -- Chromosome Sorting and Analysis by FACS -- Simultaneous Five-Six Color Multiparameter Analysis.
520 _aIn Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aJaroszeski, Mark J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHeller, Richard.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896033542
776 0 8 _iPrinted edition:
_z9780896035386
776 0 8 _iPrinted edition:
_z9781489942685
776 0 8 _iPrinted edition:
_z9781489942692
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v91
856 4 0 _uhttps://doi.org/10.1385/0896033546
912 _aZDB-2-PRO
999 _c7494
_d7494