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020 _a9781071651506
_9978-1-0716-5150-6
024 7 _a10.1007/978-1-0716-5150-6
_2doi
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_2bicssc
072 7 _aSCI017000
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082 0 4 _a571.6
_223
245 1 0 _aFluorescence In Situ Hybridization (FISH)
_h[electronic resource] :
_bApplication Guide /
_cedited by Thomas Liehr.
250 _a3rd ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXXI, 727 p. 100 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aBackground -- Microscopy and imaging: Visualization matters -- Optical filters and light sources for FISH -- Automation of FISH-procedure and evaluation -- Classification of FISH probes -- Commercial FISH probes -- Generation of painting probes from flow-sorted and microdissected chromosomes -- FISH-microdissection -- Homemade locus-specific FISH probes: Bacterial artificial chromosomes -- The standard FISH procedure -- Microwave treatment for better FISH results in a shorter time -- FISH with and without COT DNA -- One-day FISH procedure -- Quantitative FISH (Q-FISH) -- Telomere length measurement by FISH: Image processing techniques -- Directional Genomic Hybridization (dGH) for high-resolution detection of intrachromosomal rearrangements -- The replicative detargeting FISH (ReD-FISH) technique in studies of telomere replication -- Pre- and postnatal diagnostics and research on peripheral blood, bone marrow chorion, amniocytes, and fibroblasts -- Application of FISH to previously GTG-banded and/or embedded cytogenetic slides -- FISH in native amniocytes -- Tumorcytogenetic diagnostics and research on blood and bone marrow smears or effusions -- Characterization of mosaicism in different easy-to-acquire body tissues such as buccal smears, skin abrasions, hair root cells or urine -- Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction -- FISH on sperm, spermatocytes and oocytes -- Fluorescence in situ hybridization on DNA fibers: Molecular combing -- Two- to three-color FISH -- Multiplex FISH and spectral karyotyping -- FISH banding techniques -- Centromere directed M-FISH approaches (cenM-FISH) -- Heterochromatin directed M-FISH (HCM-FISH) -- FISH for repeatedly observed euchromatic variants -- Subtelomeric and/or subcentromeric probe sets -- Bar-coding is more actual than ever -- FISH for repeatedly observed constitutional rearrangements -- Parental origin determination FISH: Pod-FISH -- Simultaneous FISH and fluorescence immunostaining for DNA–protein co-detection -- CENP-antibodies used additionally to FISH -- X-inactivation accessed by FISH and EDU-immunostaining -- Immuno-FISH and microspreading technique for synaptonemal complex studies -- Yeast chromosomes revealed by immuno FISH -- Interphase FISH in diagnostics -- Interphase FISH for detection of chromosomal mosaicism and instability -- Comet-FISH -- Micronucleus FISH -- Three-dimensional interphase analysis using suspension FISH -- 3D DNA-FISH protocol for the study of the DUX gene in preimplantation bovine embryos -- Animal probes and Zoo-FISH -- Three-dimensional co-immuno and RNA-fluorescence in situ hybridization in preimplantation mouse embryos -- FISH in bird chromosomes: An updated protocol and best practices -- An updated standard protocol for FISH mapping in fish species (fish-FISH) -- FISH in lampbrush-chromosomes -- General protocol for FISH on insect chromosomes -- FISH in Drosophila -- FISH on insect cells transfected with heterologous DNA – a single cell directed approach -- Fluorescence in situ hybridization for plants: Methods of chromosomal preparation -- Mitochondrial DNA (MtDNA) detection by FISH -- Advances in the application of FISH in food microbiology -- Evaluation of polymicrobial involvement using Fluorescence In Situ Hybridization (FISH) in clinical practice -- Comparative genomic hybridization (CGH) and microdissection-based CGH (micro-CGH) in human -- Comparative genomic hybridization (CGH) in animals -- Breakpoint mapping of balanced chromosomal rearrangements using array-CGH with microdissection-derived FISH probes -- Array CGH -- Chromosome microarray results to be verified by FISH -- Sequencing of microdissection derived FISH-probes -- Next generation sequencing should consider molecular cytogenetic results -- Optical genome mapping results need to be verified by FISH -- Application of ALFA-tag signal amplification to expand the CRISPR-based DNA imaging toolkit.
520 _aThis third edition provides protocols on fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches. Chapters provide new and updated methods on probe, cell types, tissues, organisms, plants, microorganisms, multicolor FISH procedures, chromosomes, breakpoints, cryptic aberrations, nuclear architecture, epigenetic changes, and comparative genomic hybridization studies, immunostaining, single cell electrophoresis, microdissection, sequencing, and CRISP/Cas9 technology. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH): Application Guide, Thid Edition aims to be useful to researchers just starting to study FISHers and for FISH experts.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aMedical genetics.
650 1 4 _aCell Biology.
650 2 4 _aMedical Genetics.
700 1 _aLiehr, Thomas.
_eeditor.
_0(orcid)0000-0003-1672-3054
_1https://orcid.org/0000-0003-1672-3054
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071651490
776 0 8 _iPrinted edition:
_z9781071651513
776 0 8 _iPrinted edition:
_z9781071651520
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5150-6
912 _aZDB-2-PRO
999 _c7471
_d7471