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020 _a9781607619505
_9978-1-60761-950-5
024 7 _a10.1007/978-1-60761-950-5
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aLight Microscopy
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Hélio Chiarini-Garcia, Rossana C. N. Melo.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aX, 204 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v689
505 0 _aGlycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model -- Histological processing of teeth and periodontal tissues for light microscopy analysis -- Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding? -- Image cytometry: nuclear and chromosomal DNA quantification -- Histological approaches to study tissue parasitism during Trypanosoma cruzi infection -- Intravital microscopy to study leukocyte recruitment in vivo -- Introduction to fluorescence microscopy -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals -- Imaging lipid bodies within leukocytes with different light microscopy techniques -- EicosaCell – An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer -- Imaging calcium sparks in cardiac myocytes -- Light microscopy in aquatic ecology: methods for plankton communities studies -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples.
520 _aOf all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aChiarini-Garcia, Hélio.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMelo, Rossana C. N.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607619499
776 0 8 _iPrinted edition:
_z9781607619512
776 0 8 _iPrinted edition:
_z9781493957132
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v689
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-950-5
912 _aZDB-2-PRO
999 _c7451
_d7451