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001 978-1-59259-366-8
003 DE-He213
005 20260610083703.0
007 cr nn 008mamaa
008 100301s2003 xxu| s |||| 0|eng d
020 _a9781592593668
_9978-1-59259-366-8
024 7 _a10.1385/1592593666
_2doi
050 4 _aRD701-811
072 7 _aMNS
_2bicssc
072 7 _aMED065000
_2bisacsh
072 7 _aMNS
_2thema
082 0 4 _a616.7
_223
245 1 0 _aBone Research Protocols
_h[electronic resource] /
_cedited by Miep H. Helfrich, Stuart H. Ralston.
250 _a1st ed. 2003.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2003.
300 _aXIV, 450 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v80
505 0 _aCulture of Osteolblasts and Osteocytes -- Human Osteoblast Culture -- Osteoblast Isolation from Murine Calvariae and Long Bones -- Mineralizing Fibroblast-Colony-Forming Assays -- Osteocyte Isolation and Culture -- Culture of Osteoclasts -- Isolated Osteoclast Cultures -- Primary Isolation and Culture of Chicken Osteoclasts -- Isolation and Purification of Rabbit Osteoclasts -- Generating Human Osteoclasts from Peripheral Blood -- Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood -- Generating Murine Osteoclasts from Bone Marrow -- Osteoclast Formation in the Mouse Coculture Assay -- RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells -- Bone Organ Culture -- Analysis of Osteoclast Function in Mouse Calvarial Cultures -- Assessing Bone Formation Using Mouse Calvarial Organ Cultures -- Histological and Ultrastructural Techniques -- In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections -- Techniques for the Study of Apoptosis in Bone -- Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry -- Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections -- Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy -- Bone Histomorphometry -- Transmission Electron Microscopy of Bone -- Scanning Electron Microscopy of Bone -- Imaging Techniques -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- In Vivo Techniques -- Studies of Local Bone Remodeling -- Inflammation-Induced Osteoporosis -- Ovariectomy and Estrogen Replacement in Rodents -- Mechanical Loading Techniques -- Mechanical Testing of Bone Ex Vivo -- Bone Cell Responses to Fluid Flow -- Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models -- Molecular Analysis -- Extraction of Nucleic Acids from Bone -- Analysis of Gene Expression in Bone by Quantitative RT-PCR.
520 _aThe past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism.
650 0 _aOrthopedics.
650 1 4 _aOrthopaedics.
700 1 _aHelfrich, Miep H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRalston, Stuart H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588290441
776 0 8 _iPrinted edition:
_z9781617373213
776 0 8 _iPrinted edition:
_z9781489938527
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v80
856 4 0 _uhttps://doi.org/10.1385/1592593666
912 _aZDB-2-PRO
999 _c7430
_d7430