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003 DE-He213
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020 _a9781592590513
_9978-1-59259-051-3
024 7 _a10.1385/1592590519
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aCytoskeleton Methods and Protocols
_h[electronic resource] /
_cedited by Ray H. Gavin.
250 _a1st ed. 2001.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2001.
300 _aXIV, 288 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v161
505 0 _aIdentification of Cytoskeleton Proteins -- Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments -- Microsequencing of Myosins for PCR Primer Design -- Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena -- Microscopy Applications -- Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function -- Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility -- Evaluation of Individual-Cell Motility -- Evaluation of Cell Morphology -- A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena -- Reagents for Studying Cytoskeleton Protein Function -- Jasplakinolide -- Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteins -- Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy -- Tat-Mediated Delivery of Antibodies into Cultured Cells -- Cytoskeleton Dynamics -- Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein -- Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics -- Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics -- Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function -- Chromatophores as Tools for the Study of Organelle Transport -- Centriole Duplication, Centrosome Maturation and Spindle Assembly in Lysates of Spisula solidissima Oocytes -- XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate Filament Interactions -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic Approaches for Studies of Cytoskeleton Protein Function -- A Yeast Two-Hybrid Approach for Probing Cytoskeletal Protein Interactions -- Manipulating Dynein Genes in Tetrahymena -- Functional Analysis of CytoskeletalComponents in the Developing Visual System of Drosophila.
520 _aMany recent advances in experimental instrumentation, reagents, and imaging technology have dramatically expanded the range of tools available for the study of the cytoskeleton. In Cytoskeleton Methods and Protocols, Ray Gavin brings together an international panel of experienced researchers to detail the readily reproducible methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. Each protocol contains proven step-by-step instructions that enable both the novice and experienced researcher to achieve successful experimental results. The protocols utilize diverse model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional, often unpublished, information that can make the difference between success and failure. State-of-the-art and highly practical, Cytoskeleton Methods and Protocols makes available a diverse collection of powerful experimental systems and tools for successfully studying cytoskeleton structure and function.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aGavin, Ray H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896037717
776 0 8 _iPrinted edition:
_z9781617371813
776 0 8 _iPrinted edition:
_z9781489942180
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v161
856 4 0 _uhttps://doi.org/10.1385/1592590519
912 _aZDB-2-PRO
999 _c7428
_d7428