000 05387nam a22005055i 4500
001 978-1-59259-571-6
003 DE-He213
005 20260610083703.0
007 cr nn 008mamaa
008 100301s1998 xxu| s |||| 0|eng d
020 _a9781592595716
_9978-1-59259-571-6
024 7 _a10.1385/0896033929
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aCombinatorial Peptide Library Protocols
_h[electronic resource] /
_cedited by Shmuel Cabilly.
250 _a1st ed. 1998.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1998.
300 _aXIII, 313 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v87
505 0 _aSynthesis of a One-Bead One-Compound Combinatorial Peptide Library -- Enzyme-Linked Calorimetric Screening of a One-Bead One-Compound Combinatorial Library -- Synthesis and Screening of Positional Scanning Combinatorial Libraries -- Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports -- Peralkylation -- to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition -- Preparation of Biocompatible Resins for Library Syntheses -- Intramolecular Fluorescence-Quenched Substrate Libraries -- The Solid-Phase Enzyme Inhibitor Library Assay -- Determination of Peptide Substrate Motifs for Protein Kinases Using a “One-Bead One-Compound” Combinatorial Library Approach -- The Use of Peptide Library for the Determination of Kinase Peptide Substrates -- Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method) -- Generation of Multiuse Peptide Libraries for Functional Screenings -- Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay -- The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries -- Construction and Use of a 20-mer Phage Display Epitope Library -- Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII -- Conformational Mimicry Through Random Constraints Plus Affinity Selection -- The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells -- Screening Phage Display Peptide Libraries on Nitrocellulose Membranes -- Identification of Disease-Specific Epitopes -- Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas -- Major Histocompatibility Complex Allele-Specific Peptide Librariesand Identification of T-Cell Mimotopes -- Phage Display of Peptide Libraries on Protein Scaffolds -- Displaying Libraries of Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions -- Combinatorial Peptide Library as an Immunogen.
520 _aShmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. Combinatorial Peptide Library Protocols offers novice and experienced investigators alike the ability to select molecules from a randomized pool having specific biological activities. Its state-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aCabilly, Shmuel.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896033924
776 0 8 _iPrinted edition:
_z9781617370229
776 0 8 _iPrinted edition:
_z9781489942937
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v87
856 4 0 _uhttps://doi.org/10.1385/0896033929
912 _aZDB-2-PRO
999 _c7392
_d7392