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020 _a9781617790089
_9978-1-61779-008-9
024 7 _a10.1007/978-1-61779-008-9
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 4 _aThe Plant Cell Wall
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Zoë Popper.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXIII, 310 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v715
505 0 _aPlant Tissue Cultures -- Computerized Molecular Modelling of Carbohydrates -- Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS -- High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors -- Carbohydrate Gel Electrophoresis -- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence -- Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls -- Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays -- Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls -- Analyzing Cellulose Biosynthesis with Confocal Microscopy -- Visual Mapping of Cell Wall Biosynthesis -- Atomic Force Microscopy of Plant Cell Walls -- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls -- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation -- Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis -- New Insights into the Control of Cell Growth -- Extraction and Detection of Arabinogalactan Proteins -- Characterization of the Plant Cell Wall Proteome Using High Throughput Screens -- Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens -- Measuring In vitro Extensibility of Growing Plant Cell Walls.
520 _aThe cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.  The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
650 0 _aBotany.
650 0 _aPlant physiology.
650 1 4 _aPlant Science.
650 2 4 _aPlant Physiology.
700 1 _aPopper, Zoë.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617790072
776 0 8 _iPrinted edition:
_z9781617790096
776 0 8 _iPrinted edition:
_z9781493961795
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v715
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-008-9
912 _aZDB-2-PRO
999 _c7344
_d7344