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020 _a9781493924509
_9978-1-4939-2450-9
024 7 _a10.1007/978-1-4939-2450-9
_2doi
050 4 _aQR
072 7 _aPSG
_2bicssc
072 7 _aSCI045000
_2bisacsh
072 7 _aPSG
_2thema
082 0 4 _a579
_223
245 1 0 _aMycobacteria Protocols
_h[electronic resource] /
_cedited by Tanya Parish, David M. Roberts.
250 _a3rd ed. 2015.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2015.
300 _aXII, 416 p. 88 illus., 37 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1285
505 0 _aWhole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly -- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis -- RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria -- Fractionation and Analysis of Mycobacterial Proteins -- Lipid and Lipoarabinomannan Isolation and Characterization -- Metabolomics of Mycobacterium tuberculosis -- Electroporation of Mycobacteria -- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination -- Construction of Conditional Knockdown Mutants in Mycobacteria -- Mycobacterial Recombineering -- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria -- Genetic Dissection of Mycobacterial Biofilms -- Measuring Efflux and Permeability in Mycobacteria -- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy -- Antimicrobial Susceptibility Testing for Mycobacterium sp. -- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis -- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis -- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis -- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis -- Macrophage Infection Models for Mycobacterium tuberculosis -- Infection of Human Neutrophils to Study Virulence Properties of Mycobacteriumtuberculosis -- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids -- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum -- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  .
520 _aUpdated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.  Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.  Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.
650 0 _aMicrobiology.
650 1 4 _aMicrobiology.
700 1 _aParish, Tanya.
_eeditor.
_0(orcid)0000-0001-7507-0423
_1https://orcid.org/0000-0001-7507-0423
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRoberts, David M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493924493
776 0 8 _iPrinted edition:
_z9781493924516
776 0 8 _iPrinted edition:
_z9781493955824
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1285
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-2450-9
912 _aZDB-2-PRO
999 _c7340
_d7340