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003 DE-He213
005 20260610083701.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592592623
_9978-1-59259-262-3
024 7 _a10.1385/0896035182
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aQuantitative PCR Protocols
_h[electronic resource] /
_cedited by Bernd Kochanowski, Udo Reischl.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _a320 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v26
505 0 _aReviews -- Quantitative PCR -- General Principles of Quantitative PCR -- Effects of Collection, Processing, and Storage on RNA Detection and Quantification -- Quantitative RT-PCR -- Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard -- Comparison of Competitive PCR and Positive Control-Based PCR -- Protocols -- End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA -- Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence -- Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid Chromatography (HPLC) -- Quantifying Amplicons with ELISA -- Competitive PCR Quantitation Utilizing a Microtiter Plate Based Format for the Detection of PCR Products -- Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Antisense Competitors -- Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA) -- Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene -- Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing -- High Resolution PCR Quantitation by AmpliSensor Assay -- Construction of Polycompetitors for Competitive PCR -- Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA -- A Stochastic PCR Approach for RNA Quantification in Multiple Samples -- Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes.
520 _aIn Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aKochanowski, Bernd.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aReischl, Udo.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035188
776 0 8 _iPrinted edition:
_z9781489942579
776 0 8 _iPrinted edition:
_z9781489942586
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v26
856 4 0 _uhttps://doi.org/10.1385/0896035182
912 _aZDB-2-PRO
999 _c7282
_d7282