000 03430nam a22004815i 4500
001 978-1-59259-276-0
003 DE-He213
005 20260610083700.0
007 cr nn 008mamaa
008 100301s2002 xxu| s |||| 0|eng d
020 _a9781592592760
_9978-1-59259-276-0
024 7 _a10.1385/1592592767
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aPatch-Clamp Analysis
_h[electronic resource] :
_bAdvanced Techniques /
_cedited by Wolfgang Walz, Alan A. Boulton, Glen B. Baker.
250 _a1st ed. 2002.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2002.
300 _aXII, 345 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v35
505 0 _aTechnology of Patch-Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Single-Channel Recording -- Combined Fluorometric and Electrophysiological Recordings -- Patch-Clamp Techniques Applied To Brain Slices -- Perforated Patch-Clamp Technique -- Fast-Drug Application -- Pipet Perfusion Methods -- Patch-Cram Detection of Cyclic GMP in Intact Cells -- Loose-Patch Voltage-Clamp Technique -- Recording from Macropatches -- Combining Patch-Clamp Techniques with RT-PCR.
520 _aThe use of the patch pipet has moved from a specialized to a mainstream method that can be combined with other techniques to perform complex and novel operations. In Patch-Clamp Analysis: Advanced Techniques, Wolfgang Walz and his colleagues bring together a team of leading academic researchers to describe in reproducible detail all the most important patch-clamp methods. Beginning with updated basic patch-clamp techniques, these hands-on experts present the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose-patch, the perforated-patch, and the patch-cram detection technique. Coverage of these advanced techniques combines their molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques. All the methods included describe the theoretical background, offer complete startup help, and provide tips on solving common problems and analyzing data. Comprehensive and highly practical, Patch-Clamp Analysis: Advanced Techniques surveys all the major applications that involve patch pipets and sets the stage for the emergence of today's new applications involving genomics, proteomics, and sophisticated imaging techniques.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aWalz, Wolfgang.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBoulton, Alan A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBaker, Glen B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588290137
776 0 8 _iPrinted edition:
_z9781489938558
776 0 8 _iPrinted edition:
_z9781489938565
830 0 _aNeuromethods,
_x1940-6045 ;
_v35
856 4 0 _uhttps://doi.org/10.1385/1592592767
912 _aZDB-2-PRO
999 _c7256
_d7256