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001 978-1-59259-631-7
003 DE-He213
005 20260610083700.0
007 cr nn 008mamaa
008 100301s1995 xxu| s |||| 0|eng d
020 _a9781592596317
_9978-1-59259-631-7
024 7 _a10.1385/0896033112
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aPatch-Clamp Applications and Protocols
_h[electronic resource] /
_cedited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz.
250 _a1st ed. 1995.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1995.
300 _aXVI, 316 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v26
505 0 _aTechnology of Patch- Clamp Electrodes -- Whole-Cell Patch-Clamp Recordings -- Pressure/Patch-Clamp Methods -- Cell-Free Ion-Channel Recording -- Perfusion of Patch Pipets -- Concentration Clamp Technique -- Perforated Patch-Clamp Technique -- The Loose Patch Voltage Clamp Technique -- Patch-Clamp Recording and RT-PCR on Single Cells -- Patch-Clamp Technique in Brain Slices -- Xenopus Oocyte Microinjection and Ion-Channel Expression.
520 _aE. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aBoulton, Alan A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBaker, Glen B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWalz, Wolfgang.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896033115
776 0 8 _iPrinted edition:
_z9781489940636
776 0 8 _iPrinted edition:
_z9781489940643
830 0 _aNeuromethods,
_x1940-6045 ;
_v26
856 4 0 _uhttps://doi.org/10.1385/0896033112
912 _aZDB-2-PRO
999 _c7225
_d7225