000 04865nam a22004455i 4500
001 978-1-59259-823-6
003 DE-He213
005 20260610083659.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598236
_9978-1-59259-823-6
024 7 _a10.1385/1592598234
_2doi
050 4 _aRS1-441
072 7 _aMQP
_2bicssc
072 7 _aMED072000
_2bisacsh
072 7 _aMQP
_2thema
082 0 4 _a615.1
_223
245 1 0 _aOligonucleotide Synthesis
_h[electronic resource] :
_bMethods and Applications /
_cedited by Piet Herdewijn.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXVI, 436 p. 142 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v288
505 0 _aSynthesis of DNA Using a New Two-Step Cycle -- RNA Synthesis Using 2?-O-(Tert-Butyldimethylsilyl) Protection -- RNA Oligonucleotide Synthesis Via 5?-Silyl-2?-Orthoester Chemistry -- Dimethylthiarum Disulfide -- Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes -- Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry -- Solid-Phase Synthesis of Circular Oligonucleotides -- Locked Nucleic Acid Synthesis -- Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers -- Base-Modified Oligonucleotides With Increased Duplex Stability -- of Hypermodified Nucleotides in RNA -- Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis -- Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms -- Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips -- Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides -- Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis -- Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays -- High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing -- Nonenzymatic Template-Directed RNA Synthesis -- DNase I Footprinting of Small Molecule Binding Sites on DNA -- Gene Targeting Using Peptide Nucleic Acid -- Nucleic Acid Library Construction Using Synthetic DNA Constructs -- In Vitro Selection From Combinatorial Nucleic Acid Libraries -- In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins -- Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism.
520 _aNucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.
650 0 _aPharmacy.
650 1 4 _aPharmacy.
700 1 _aHerdewijn, Piet.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292339
776 0 8 _iPrinted edition:
_z9781617374418
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v288
856 4 0 _uhttps://doi.org/10.1385/1592598234
912 _aZDB-2-PRO
999 _c7162
_d7162