000 04179nam a22004815i 4500
001 978-1-59259-487-0
003 DE-He213
005 20260610083659.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592594870
_9978-1-59259-487-0
024 7 _a10.1385/0896035662
_2doi
050 4 _aQH541.15.M64
072 7 _aTQD
_2bicssc
072 7 _aSCI026000
_2bisacsh
072 7 _aTQD
_2thema
082 0 4 _a363.7063
_223
245 1 0 _aEnvironmental Monitoring of Bacteria
_h[electronic resource] /
_cedited by Clive Edwards.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aX, 333 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v12
505 0 _aSome Problems Posed by Natural Environments for Monitoring Microorganisms -- Sampling Sediment and Soil -- Sampling Water Bodies -- Dielectrophoresis -- Flow Cytometry and Cell Sorting -- Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments -- DNA Extraction from Natural Environments -- Automated Sequencing of DNA Retrieved from Environmental Samples -- Analysis of DNA Sequences -- Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments -- Recovery and Analysis of Ribosomal RNA Sequences from the Environment -- Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology -- Reporter Gene Expression for Monitoring Microorganisms in the Environment -- Characterizing Microorganisms in the Environment by Fatty Acid Analysis -- Fluorescent In Situ Hybridization and the Analysis of the Single Cell -- Specific Detection, Viability Assessment, and Macromolecular Staining of Bacteria for Flow Cytometry -- Confocal Laser Scanning Microscopy of Environmental Samples -- Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry -- Experimental Biofilms and Their Applications in the Study of Environmental Processes -- Establishment of Experimental Biofilms Using the Modified Robbins Device and Flow Cells.
520 _aIn Environmental Monitoring of Bacteria, Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing, and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional methods. In addition to providing many eminently reproducible methods, the distinguished contributors review the problems of monitoring bacteria in natural environments and discuss the principles by which the methods work. Taking full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteria gives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level.
650 0 _aEnvironmental monitoring.
650 0 _aBacteria.
650 1 4 _aEnvironmental Monitoring.
650 2 4 _aBacteria.
700 1 _aEdwards, Clive.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035669
776 0 8 _iPrinted edition:
_z9781617370779
776 0 8 _iPrinted edition:
_z9781489943231
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v12
856 4 0 _uhttps://doi.org/10.1385/0896035662
912 _aZDB-2-PRO
999 _c7159
_d7159