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020 _a9781617791239
_9978-1-61779-123-9
024 7 _a10.1007/978-1-61779-123-9
_2doi
050 4 _aQK981-981.7
072 7 _aPST
_2bicssc
072 7 _aPSAK
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072 7 _aSCI011000
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082 0 4 _a581.35
_223
245 1 0 _aRNAi and Plant Gene Function Analysis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Hiroaki Kodama, Atsushi Komamine.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aX, 244 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v744
505 0 _aOverview of Plant RNAi -- Caveat of RNAi in Plants: the Off-Target Effect -- Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies -- Heat-Inducible RNAi for Gene Functional Analysis in Plants -- Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens -- Virus-Induced Gene Silencing in Ornamental Plants -- Local RNA Silencing Mediated by Agroinfiltration -- Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana -- Detection of Long and Short Double-Stranded RNAs -- Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs -- Large Scale Sequencing of Plant Small RNAs -- Computational Prediction of Plant miRNA Targets -- Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants -- Using Nuclear Run-On Transcription Assays in RNAi Studies -- Proteomic Analysis of RNA-Interference Induced Knockdown Plant -- Comparative Analysis of Phosphoprotein Expression using 2D-DIGE.
520 _aThe use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.
650 0 _aPlant genetics.
650 1 4 _aPlant Genetics.
700 1 _aKodama, Hiroaki.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aKomamine, Atsushi.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617791222
776 0 8 _iPrinted edition:
_z9781617791246
776 0 8 _iPrinted edition:
_z9781627039321
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v744
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-123-9
912 _aZDB-2-PRO
999 _c7137
_d7137