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008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598670
_9978-1-59259-867-0
024 7 _a10.1385/1592598676
_2doi
050 4 _aRA1001-1171
072 7 _aMMQ
_2bicssc
072 7 _aMED030000
_2bisacsh
072 7 _aMKT
_2thema
082 0 4 _a614.1
_223
245 1 0 _aForensic DNA Typing Protocols
_h[electronic resource] /
_cedited by Angel Carracedo.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aX, 280 p. 81 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v297
505 0 _aForensic DNA-Typing Technologies -- DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR -- A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples -- Species Determination -- Constructing STR Multiplex Assays -- Y Chromosome STR Typing -- Using Online Databases for Developing SNP Markers of Forensic Interest -- SNP Typing in Forensic Genetics -- Genotyping SNPs With the LightCycler -- Universal Tag Arrays in Forensic SNP Analysis -- SNP Typing on the NanoChip Electronic Microarray -- Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS -- Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing -- SNaPshot Typing of Mitochondrial DNA Coding Region Variants -- Typing of Y Chromosome SNPs With Multiplex PCR Methods -- Y Chromosome SNP Analysis Using the Single-Base Extension -- SNaPshot for Pharmacogenetics by Minisequencing -- Methods for the Study of Ancient DNA -- Protocols for Ancient DNA Typing.
520 _aIn recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.
650 0 _aMedical jurisprudence.
650 1 4 _aForensic Medicine.
700 1 _aCarracedo, Angel.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292643
776 0 8 _iPrinted edition:
_z9781617374623
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v297
856 4 0 _uhttps://doi.org/10.1385/1592598676
912 _aZDB-2-PRO
999 _c7095
_d7095