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020 _a9781607616467
_9978-1-60761-646-7
024 7 _a10.1007/978-1-60761-646-7
_2doi
050 4 _aQK981-981.7
072 7 _aPST
_2bicssc
072 7 _aPSAK
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082 0 4 _a581.35
_223
245 1 0 _aPlant Epigenetics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Igor Kovalchuk, Franz J. Zemp.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aX, 273 p. 51 illus., 12 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v631
505 0 _aAnalysis of DNA Methylation in Plants by Bisulfite Sequencing -- Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE -- Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay -- Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis of DNA Methylation in Plants -- Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism -- Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes -- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- qRT-PCR of Small RNAs -- Cloning New Small RNA Sequences -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis -- Metaanalysis of ChIP-chip Data -- Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein–DNA Binding Analyses in Arabidopsis -- cDNA Libraries for Virus-Induced Gene Silencing -- Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay -- Reporter Gene-Based Recombination Lines for Studies of Genome Stability -- Plant Transgenesis.
520 _aThe past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.
650 0 _aPlant genetics.
650 0 _aBiochemistry.
650 0 _aBotany.
650 0 _aMolecular genetics.
650 1 4 _aPlant Genetics.
650 2 4 _aBiochemistry.
650 2 4 _aPlant Science.
650 2 4 _aMolecular Genetics.
700 1 _aKovalchuk, Igor.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZemp, Franz J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607616450
776 0 8 _iPrinted edition:
_z9781607616504
776 0 8 _iPrinted edition:
_z9781493957569
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v631
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-646-7
912 _aZDB-2-PRO
999 _c7076
_d7076