000 04015nam a22004455i 4500
001 978-1-59259-846-5
003 DE-He213
005 20260610083657.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598465
_9978-1-59259-846-5
024 7 _a10.1385/1592598463
_2doi
050 4 _aQR
072 7 _aPSG
_2bicssc
072 7 _aSCI045000
_2bisacsh
072 7 _aPSG
_2thema
082 0 4 _a579
_223
245 1 0 _aMicrobial Enzymes and Biotransformations
_h[electronic resource] /
_cedited by Jose Luis Barredo.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXII, 320 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v17
505 0 _aMicrobial Cells and Enzymes A Century of Progress -- Enzyme Biosensors -- Directed Evolution of Enzymes -- L-Glutaminase as a Therapeutic Enzyme of Microbial Origin -- Enzymatic Production of D-Amino Acids -- Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides -- Food-Grade Corynebacteria for Enzyme Production -- Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase -- Enzymes in Modern Detergents -- Microbial Proteases -- Proteases Produced by Halophilic Bacteria and Archaea -- Microbial Pectinases -- Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes -- Glucose Dehydrogenase for the Regeneration of NADPH and NADH -- Acetate Kinase From Methanosarcina thermophila, a Key Enzyme for Methanogenesis -- Immobilization of Enzymes by Covalent Attachment -- Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads -- Methods for Bioencapsulation of Enzymes and Cells.
520 _aThe application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.
650 0 _aMicrobiology.
650 1 4 _aMicrobiology.
700 1 _aBarredo, Jose Luis.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292537
776 0 8 _iPrinted edition:
_z9781617374548
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v17
856 4 0 _uhttps://doi.org/10.1385/1592598463
912 _aZDB-2-PRO
999 _c7072
_d7072