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003 DE-He213
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020 _a9781607618638
_9978-1-60761-863-8
024 7 _a10.1007/978-1-60761-863-8
_2doi
050 4 _aRC261-271
072 7 _aMJCL
_2bicssc
072 7 _aMED062000
_2bisacsh
072 7 _aMJCL
_2thema
082 0 4 _a571.978
_223
082 0 4 _a616.994
_223
245 1 0 _aMicroRNA and Cancer
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Wei Wu.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aX, 273 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v676
505 0 _aMicroRNA Biogenesis and Cancer -- Computational Identification of MiRNAs Involved in Cancer -- The Principles of MiRNA-Masking Antisense Oligonucleotides Technology -- The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology -- Modulation of MicroRNAs for Potential Cancer Therapeutics -- Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In situ Hybridization -- MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference -- MiRNA Profiling on High Throughput OpenArray™ System -- Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs -- High Throughput and Reliable Protocols for Animal microRNA Library Cloning -- MicroRNA Regulation of Growth Factor Receptor Signalling in Human Cancer Cells -- Epigenetic Regulation of MicroRNA Expression in Cancer -- In vitro Functional Study of miR-126 in Leukemia -- Prediction of the Biological Effect of Polymorphisms within MicroRNA Binding Sites -- The Guideline of the Design and Validation of MiRNA Mimics -- Analysis of Targets and Functions Co-Regulated by MicroRNAs -- Utilization of SSCprofiler to Predict a New MiRNA Gene -- MicroRNA-Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing.
520 _aThe tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: • Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment • Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study • Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs • Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.
650 0 _aCancer.
650 0 _aMedical genetics.
650 1 4 _aCancer Biology.
650 2 4 _aMedical Genetics.
700 1 _aWu, Wei.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607618621
776 0 8 _iPrinted edition:
_z9781607618645
776 0 8 _iPrinted edition:
_z9781493957743
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v676
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-863-8
912 _aZDB-2-PRO
999 _c7052
_d7052