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008 100301s2007 xxu| s |||| 0|eng d
020 _a9781597453998
_9978-1-59745-399-8
024 7 _a10.1007/978-1-59745-399-8
_2doi
050 4 _aTP248.13-248.65
072 7 _aTCB
_2bicssc
072 7 _aSCI010000
_2bisacsh
072 7 _aTCB
_2thema
082 0 4 _a660.6
_223
245 1 0 _aAnimal Cell Biotechnology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Ralf Pörtner.
250 _a2nd ed. 2007.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2007.
300 _aXV, 512 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v24
505 0 _aBasic Techniques for Establishing Production Cell Lines -- Transduction Technologies -- Cells for Gene Therapy and Vector Production -- Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments -- DNA Fingerprinting and Characterization of Animal Cell Lines -- Basic Cultivation Techniques -- Microcarrier Cell Culture Technology -- Cell Encapsulation -- Tools for High-Throughput Medium and Process Optimization -- Cell Characterization and Analysis -- Cell Counting and Viability Measurements -- Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry -- Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors -- Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture -- Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation -- Measurement of Apoptosis in Cell Culture -- Metabolic Flux Estimation in Mammalian Cell Cultures -- Special Cultivation Techniques -- Disposable Bioreactors for Inoculum Production and Protein Expression -- Hollow Fiber Cell Culture -- Cultivation of Mammalian Cells in Fixed-Bed Reactors -- Configuration of Bioreactors -- Downstream Techniques -- Membrane Filtration in Animal Cell Culture -- Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins -- Special Applications -- Vaccine Production -- Retrovirus Production and Characterization -- Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production.
520 _aAnimal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
650 0 _aBiotechnology.
650 0 _aCytology.
650 0 _aGenetics.
650 0 _aPharmacology.
650 1 4 _aBiotechnology.
650 2 4 _aCell Biology.
650 2 4 _aGenetics and Genomics.
650 2 4 _aPharmacology.
700 1 _aPörtner, Ralf.
_eeditor.
_0(orcid)0000-0003-1163-9718
_1https://orcid.org/0000-0003-1163-9718
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588296603
776 0 8 _iPrinted edition:
_z9781627039635
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v24
856 4 0 _uhttps://doi.org/10.1007/978-1-59745-399-8
912 _aZDB-2-PRO
999 _c6969
_d6969