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020 _a9781627035231
_9978-1-62703-523-1
024 7 _a10.1007/978-1-62703-523-1
_2doi
050 4 _aQR180-189.5
072 7 _aMJCM
_2bicssc
072 7 _aMED044000
_2bisacsh
072 7 _aMJCM
_2thema
082 0 4 _a571.96
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082 0 4 _a616.079
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245 1 4 _aThe Inflammasome
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Christine M. De Nardo, Eicke Latz.
250 _a1st ed. 2013.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2013.
300 _aXI, 225 p. 44 illus., 21 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1040
505 0 _aAssessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia -- Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP -- Assessment and Quantification of Crystal-induced Lysosomal Damage -- Assessment of Inflammasome Activation in Primary Human Immune Cells -- NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants -- Measuring Inflammasome Activation in Response to Bacterial Infection -- Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity -- ASC Speck Formation as a Read-out for Inflammasome Activation -- Immunoblotting for Active Caspase-1 --  Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1 -- Reconstituting the NLRP1 Inflammasome in vitro -- Assessing ATP Binding and Hydrolysis by NLR Proteins -- Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition -- Analysis of Microbiota Alterations in Inflammasome-deficient Mice -- Quantification of Adipose Tissue Leukocytosis in Obesity -- In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates.
520 _a Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
650 0 _aImmunology.
650 0 _aCytokines.
650 1 4 _aImmunology.
650 2 4 _aCytokines and Growth Factors.
700 1 _aDe Nardo, Christine M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLatz, Eicke.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627035224
776 0 8 _iPrinted edition:
_z9781627035248
776 0 8 _iPrinted edition:
_z9781493960118
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1040
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-523-1
912 _aZDB-2-PRO
999 _c6955
_d6955