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020 _a9781489977083
_9978-1-4899-7708-3
024 7 _a10.1007/978-1-4899-7708-3
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Epigenetics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Igor Kovalchuk.
250 _a2nd ed. 2017.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2017.
300 _aX, 253 p. 51 illus., 32 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1456
505 0 _aChromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis -- Chromatin Conformation Capture-Based Analysis of Nuclear Architecture -- Metaanalysis of Genome-Wide Chromatin Data -- Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide Probes -- COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns -- Analysis of Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis Of DNA Methylation In Plants -- Analysis of DNA Hydroxymethylation In Arabidopsis Using Colorimetric Assay -- Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP) -- Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized Genomes -- Analysis of Small RNA PopulationsUsing Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- Stem-Loop Qrt-PCR for the Detection of Plant Micrornas -- Profiling New Small RNA Sequences -- Small RNA Library Preparation and Illumina Sequencing in Plants -- Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow -- Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression using Virus-Induced Gene Silencing -- The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks -- Profiling Transposable Elements and their Epigenetic Effects in Non-Model Species.
520 _aThis volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. .
650 0 _aBotany.
650 1 4 _aPlant Science.
700 1 _aKovalchuk, Igor.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781489977069
776 0 8 _iPrinted edition:
_z9781489977076
776 0 8 _iPrinted edition:
_z9781493979561
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1456
856 4 0 _uhttps://doi.org/10.1007/978-1-4899-7708-3
912 _aZDB-2-PRO
999 _c6921
_d6921