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020 _a9781603270052
_9978-1-60327-005-2
024 7 _a10.1007/978-1-60327-005-2
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
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245 1 0 _aPlant MicroRNAs
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Blake C. Meyers, Pamela J. Green.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aX, 261 p. 46 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v592
505 0 _aPiecing the Puzzle Together: Genetic Requirements for miRNA Biogenesis in Arabidopsis thaliana -- Prediction of Plant miRNA Genes -- Methods for Isolation of Total RNA to Recover miRNAs and Other Small RNAs from Diverse Species -- miRNA Target Prediction in Plants -- A Method to Discover Phased siRNA Loci -- Directed Gene Silencing with Artificial MicroRNAs -- Bioinformatics Analysis of Small RNAs in Plants Using Next Generation Sequencing Technologies -- High-Throughput Approaches for miRNA Expression Analysis -- In Situ Detection of miRNAs Using LNA Probes -- Analysis of miRNA Modifications -- MicroRNA Promoter Analysis -- Computational Methods for Comparative Analysis of Plant Small RNAs -- Biotic Stress-Associated microRNAs: Identification, Detection, Regulation, and Functional Analysis -- Abiotic Stress-Associated miRNAs: Detection and Functional Analysis -- Processing of miRNA Precursors -- Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs -- Transient Assays for the Analysis of miRNA Processing and Function.
520 _aMicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
650 0 _aBiochemistry.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aMedical genetics.
650 1 4 _aBiochemistry.
650 2 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aMedical Genetics.
700 1 _aMeyers, Blake C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGreen, Pamela J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781603270045
776 0 8 _iPrinted edition:
_z9781607614142
776 0 8 _iPrinted edition:
_z9781617796531
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v592
856 4 0 _uhttps://doi.org/10.1007/978-1-60327-005-2
912 _aZDB-2-PRO
999 _c6891
_d6891