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020 _a9781592591121
_9978-1-59259-112-1
024 7 _a10.1385/1592591124
_2doi
050 4 _aTP248.13-.65
072 7 _aTCB
_2bicssc
072 7 _aTEC009010
_2bisacsh
072 7 _aTCB
_2thema
082 0 4 _a660.63
_223
245 1 0 _aEnzymes in Nonaqueous Solvents
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Evgeny N. Vulfson.
250 _a1st ed. 2001.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2001.
300 _aXIX, 679 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Biotechnology,
_x1940-607X ;
_v15
505 0 _aControl of Enzyme Activity in Nonaqueous Solvents -- Salt-Induced Activation of Enzymes in Organic Solvents -- Imprinting Enzymes for Use in Organic Media -- Entrapment of Biocatalysts by Prepolymer Methods -- Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformations -- Immobilization of Lipases on Hydrogels -- Polyethylene Glycol-Modified Enzymes in Hydrophobic Media -- Chemical Modification of Lipase for Use in Ester Synthesis -- Preparation and Properties in Organic Solvents of Noncovalent PEG-Enzyme Complexes -- Preparation of a Lipid-Coated Enzyme and Activity for Reverse Hydrolysis Reactions in Homogeneous Organic Media -- Very High Activity Biocatalysts for Low-Water Systems -- Methods for Measurement and Control of Water in Nonaqueous Biocatalysis -- Water Activity Control in Organic Media by Equilibration Through Membranes -- Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Media -- Immobilization of Enzymes and Control of Water Activity in Low-Water Media -- Enzyme Activity and Enantioselectivity Measurements in Organic Media -- Calorimetric Methods in Evaluating Hydration and Solvation of Solid Proteins Immersed in Organic Solvents -- Detection of Structural Changes of Enzymes in Nonaqueous Media by Fluorescence and CD Spectroscopy -- The Effects of Crown Ethers on the Activity of Enzymes in Organic Solvents -- Control of Acid-Base Conditions in Low-Water Media -- Enzymatic Acylation of ?-Butylglucoside in Nonaqueous Media -- Synthetic Applications -- Choosing Hydrolases for Enantioselective Reactions Involving Alcohols Using Empirical Rules -- Candida antarctica Lipase B A Tool for the Preparation of Optically Active Alcohols -- Enantioselective Lipase-Catalyzed Transesterifications in Organic Solvents -- Pseudomonas cepacia Lipase-CatalyzedEnantioselective Acylation of 2-Substituted-1-alkanols in Organic Solvents -- Preparation of 2-, 3-, and 4-Methylcarboxylic Acids and the Corresponding Alcohols of High Enantiopurity by Lipase-Catalyzed Esterification -- Optimization of Enzymatic Enantiomeric Resolutions Through Solvent Selection -- Chemoselective Amidification of Amino-Polyols Catalyzed with Lipases in Organic Solvents -- Synthesis of Esters Catalyzed by Lipases in Water-in-Oil Microemulsions -- Enzymatic Conversion of Organosilicon Compounds in Organic Solvents -- Synthetic Applications of Enzymes in Nonaqueous Media -- Enzymes in Nonaqueous Solvents -- Interface Bioreactor -- Yeast-Mediated Reactions in Organic Solvents -- Biocatalysis in Pharmaceutical Process Development SCH56592, A Case Study -- Reaction Systems and Bioreactor Design -- Enzymatic Solid-to-Solid Peptide Synthesis -- Enzymatic Synthesis and Hydrolysis Reactions of Acylglycerols in Solvent-Free Systems -- Solid-Gas Catalysis at Controlled Water Activity -- Solvent-Free Biotransformations of Lipids -- Lipase-Catalyzed Synthesis of Sugar Fatty Acid Esters in Supercritical Carbon Dioxide -- Transformations in Frozen Aqueous Solutions Catalyzed by Hydrolytic Enzymes -- Enzymatic Synthesis of Sugar Fatty Acid Esters in Solvent-Free Media -- Biotransformations in Supersaturated Solutions -- Enzymatic Transformations in Suspensions (I) One Solid Substrate and Product -- Biotransformations in Supercritical Fluids -- Reverse Micellar Systems -- Enzymatic Transformations in Supercritical Fluids -- Enzymatic Transformations in Suspensions (II) -- Characterization and Operation of a Micellar Membrane Bioreactor -- Immobilization of Lipase Enzymes and Their Application in the Interesterification of Oils and Fats.
520 _aIn recent years, enzymatic catalysis in organic solvents-as opposed to aqueous solutions-has gained considerable attention as a powerful new approach to the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design. Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.
650 0 _aBiotechnology.
650 1 4 _aChemical Bioengineering.
700 1 _aVulfson, Evgeny N.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896039292
776 0 8 _iPrinted edition:
_z9781617372568
776 0 8 _iPrinted edition:
_z9781489942265
830 0 _aMethods in Biotechnology,
_x1940-607X ;
_v15
856 4 0 _uhttps://doi.org/10.1385/1592591124
912 _aZDB-2-PRO
999 _c6837
_d6837