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020 _a9781493919635
_9978-1-4939-1963-5
024 7 _a10.1007/978-1-4939-1963-5
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aNeural Tracing Methods
_h[electronic resource] :
_bTracing Neurons and Their Connections /
_cedited by Benjamin R. Arenkiel.
250 _a1st ed. 2015.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2015.
300 _aXII, 278 p. 52 illus., 47 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aNeuromethods,
_x1940-6045 ;
_v92
505 0 _aA Survey of Current Neuroanatomical Tracing Techniques.- Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry -- Retrograde Tract-Tracing ‘Plus’: Adding Extra Value to Retrogradely-Traced Neurons -- Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice -- Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics -- Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes -- Optogenetic Dissection of Neural Circuit Function in Behaving Animals -- Remote Control of Neural Activity Using Chemical Genetics -- Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits -- Engineered Rabies Virus for Transsynaptic Circuit Tracing -- Genetic Labeling of Synapses -- Genetic Pathways to Circuit Understanding in Drosophila.
520 _aThis volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aArenkiel, Benjamin R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493919628
776 0 8 _iPrinted edition:
_z9781493919642
776 0 8 _iPrinted edition:
_z9781493943418
830 0 _aNeuromethods,
_x1940-6045 ;
_v92
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-1963-5
912 _aZDB-2-PRO
999 _c6826
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