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020 _a9781627037150
_9978-1-62703-715-0
024 7 _a10.1007/978-1-62703-715-0
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aCereal Genomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Robert J. Henry, Agnelo Furtado.
250 _a1st ed. 2014.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2014.
300 _aX, 300 p. 39 illus., 29 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1099
505 0 _aDNA Extraction from Vegetative Tissue for Next Generation Sequencing  -- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) -- RNA Extraction from Cereal Vegetative Tissue -- RNA Extraction from Developing or Mature Wheat Seeds -- cDNA Library Preparation -- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants -- The Polymerase Chain Reaction (PCR) - General Methods -- Mutation and Mutation Screening -- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression -- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel -- Rapid Cloning of Genes and Promoters for Functional Analyses -- Genome Walking -- Functional Analysis by Protein Biochemistry -- Genomic Southern Blot analysis -- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs -- Protein Blotting-Protocol for Beginners -- Genetic Transformation of Wheat via Particle Bombardment -- Sorghum Genetic Transformation by Particle Bombardment -- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery -- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation -- Agrobacterium-mediated Transformation- Rice Transformation -- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos -- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops -- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.
520 _aIn Cereal Genomics: Methods and Protocols,  expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aBiology
_xTechnique.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aExperimental Organisms.
700 1 _aHenry, Robert J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aFurtado, Agnelo.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627037143
776 0 8 _iPrinted edition:
_z9781627037167
776 0 8 _iPrinted edition:
_z9781493960392
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1099
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-715-0
912 _aZDB-2-PRO
999 _c6810
_d6810