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020 _a9781607618171
_9978-1-60761-817-1
024 7 _a10.1007/978-1-60761-817-1
_2doi
050 4 _aQR180-189.5
072 7 _aMJCM
_2bicssc
072 7 _aMED044000
_2bisacsh
072 7 _aMJCM
_2thema
082 0 4 _a571.96
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082 0 4 _a616.079
_223
245 1 0 _aDiagnostic Virology Protocols
_h[electronic resource] /
_cedited by John R. Stephenson, Alan Warnes.
250 _a2nd ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXIII, 470 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v665
505 0 _aThe Detection of Adenoviruses -- Alphaviruses -- Detection of Human Caliciviruses in Fecal Samples by RT-PCR -- The Detection of Dengue Virus -- Detection of Enteroviruses from Clinical Specimens -- The Detection of Hepatitis Viruses -- Molecular Detection of Herpesviruses -- HIV Drug Resistance Testing -- Detection, Quantification, and Characterization of HIV/SIV -- Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Control -- Detection of Measles, Mumps, and Rubella Viruses -- Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarray -- The Detection of Parvoviruses -- The Detection and Characterization of Polioviruses -- Detection of Human-Pathogenic Poxviruses -- Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus -- Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens -- Rotaviruses -- Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR -- Detection of SARS Coronavirus -- The Detection of West Nile Virus -- Point of Care Testing: Diagnosis Outside the Virology Laboratory -- Modelling Emerging Viral Epidemics for Public Health Protection.
520 _aRecent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.
650 0 _aImmunology.
650 0 _aVirology.
650 1 4 _aImmunology.
650 2 4 _aVirology.
700 1 _aStephenson, John R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWarnes, Alan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607618164
776 0 8 _iPrinted edition:
_z9781607618188
776 0 8 _iPrinted edition:
_z9781493958634
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v665
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-817-1
912 _aZDB-2-PRO
999 _c6777
_d6777