000 05311nam a22004695i 4500
001 978-1-61779-328-8
003 DE-He213
005 20260610083652.0
007 cr nn 008mamaa
008 110912s2011 xxu| s |||| 0|eng d
020 _a9781617793288
_9978-1-61779-328-8
024 7 _a10.1007/978-1-61779-328-8
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aNeurodegeneration
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Giovanni Manfredi, Hibiki Kawamata.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXV, 512 p. 100 illus., 9 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v793
505 0 _aIntroduction to Neurodegenerative Diseases and Related Techniques -- In vivo and In vitro Determination of Cell Death Markers in Neurons -- Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods -- Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex -- Isolation and Culture of Postnatal Spinal Motoneurons -- Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration -- Neural Differentiation of Induced Pluripotent Stem Cells -- Developing Yeast Models of Human Neurodegenerative Disorders -- Modeling Dopamine Neuron Degeneration in C. elegans -- Role of the Proteasome in Fly Models of Neurodegeneration -- Modeling Neurodegenerative Diseases in Zebrafish Embryos -- Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases -- Modeling Focal Cerebral Ischemia In vivo -- Pharmacological Models of Parkison's Disease in Rodents -- Behavioral Phenotyping of Mouse Models of Neurodegeneration -- Characterization of Amyloid Deposits in Neurodegenerative Diseases -- Detection of Protein Aggregates in Neurodegenerative Diseases -- Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures -- Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium -- Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue -- Monitoring Mitophagy in Neuronal Cell Cultures -- Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging -- Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices -- Imaging Presynaptic Exocytosis in Corticostriatal Slices -- Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1 -- Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice -- Determination of Neurotransmitter Levels in Models of Parkinson’s Disease by HPLC-ECD -- Designing, Performing, and Interpreting a Microarray-based Gene Expression Study -- Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases -- Specific Gene Silencing using RNAi in Cell Culture -- Stem Cell Transplantation for Spinal Cord Neurodegeneration -- Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases.
520 _aNeurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular Biology™  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.
650 0 _aNeurosciences.
650 1 4 _aNeuroscience.
700 1 _aManfredi, Giovanni.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aKawamata, Hibiki.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617793271
776 0 8 _iPrinted edition:
_z9781617793295
776 0 8 _iPrinted edition:
_z9781493962204
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v793
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-328-8
912 _aZDB-2-PRO
999 _c6758
_d6758