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020 _a9781627032513
_9978-1-62703-251-3
024 7 _a10.1007/978-1-62703-251-3
_2doi
050 4 _aRC321-580
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
_2bisacsh
072 7 _aPSAN
_2thema
082 0 4 _a612.8
_223
245 1 0 _aDopamine
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Nadine Kabbani.
250 _a1st ed. 2013.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2013.
300 _aXI, 312 p. 78 illus., 22 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v964
505 0 _aDetection of Cell Surface Dopamine Receptors -- Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells -- Methods of Dopamine Research in Retina Cells -- Capture of D2 Dopamine Receptor Signaling Complexes in Striatal Cells for Mass Spectrometry Proteomic Analysis -- Modeling Spatial Aspects of Intracellular Dopamine Signaling -- A Biophysical Approach for the Study of Dopamine Receptor Oligomerization -- Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET (SRET) Technology -- BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling -- Dopaminergic Regulation of Dendritic Calcium: Fast Multi-Site Calcium Imaging -- Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosolic End of Transmembrane Region 6 -- A Molecular Genetic Approach to Uncovering the Differential Functions of Dopamine D2 Receptor Isoforms -- Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish -- Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila -- Regulation of Dopamine Transporter Expression by Neuronal Activity -- Monitoring Somatodendritic and Axonal Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices -- Real-Time Chemical Measurements of Dopamine Release in the Brain -- The MPTP/Probenecid Model of Progressive Parkinson’s Disease.
520 _aDopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.  In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
650 0 _aNeurosciences.
650 0 _aNeurochemistry.
650 1 4 _aNeuroscience.
650 2 4 _aNeurochemistry.
700 1 _aKabbani, Nadine.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627032506
776 0 8 _iPrinted edition:
_z9781627032520
776 0 8 _iPrinted edition:
_z9781493963331
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v964
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-251-3
912 _aZDB-2-PRO
999 _c6704
_d6704