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020 _a9781493935970
_9978-1-4939-3597-0
024 7 _a10.1007/978-1-4939-3597-0
_2doi
050 4 _aRB155-155.8
072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
_2thema
082 0 4 _a616.042
_223
245 1 0 _aForensic DNA Typing Protocols
_h[electronic resource] /
_cedited by William Goodwin.
250 _a2nd ed. 2016.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2016.
300 _aX, 298 p. 28 illus., 18 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1420
505 0 _aCollection of Samples for DNA Analysis -- Body Fluid Identification Using mRNA Profiling -- mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification -- Preservation of and DNA Extraction from Muscle Tissue -- DNA Extraction: Organic and Solid-Phase -- Extraction of DNA from Skeletal Remains -- Extraction of DNA from Human Skeletal Material -- The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis -- NucleoSpin ®XS Columns for DNA Concentration and Clean-Up -- Purification of PCR Products to Improve STR Profiles -- Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex ® Kit -- Analysis of Mitochondrial Control Region Using Sanger Sequencing -- Whole Human Mitochondrial DNA Sequencing -- In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome -- Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted Pre-Amplification and Next Generation Y-STR Amplification Systems -- Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y STRs) -- A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA -- Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry – Informative Marker Sets -- Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data -- Species Determination: The Role and Use of the Cytochrome b Gene.
520 _aThis volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science. .
650 0 _aMedical genetics.
650 0 _aMedical jurisprudence.
650 1 4 _aMedical Genetics.
650 2 4 _aForensic Medicine.
700 1 _aGoodwin, William.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493935956
776 0 8 _iPrinted edition:
_z9781493935963
776 0 8 _iPrinted edition:
_z9781493980895
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1420
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-3597-0
912 _aZDB-2-PRO
999 _c6641
_d6641