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020 _a9781627037730
_9978-1-62703-773-0
024 7 _a10.1007/978-1-62703-773-0
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
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082 0 4 _a580
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245 1 0 _aPlant Epigenetics and Epigenomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Charles Spillane, Peter C. McKeown.
250 _a1st ed. 2014.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2014.
300 _aX, 245 p. 39 illus., 12 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1112
505 0 _aLandscaping Plant Epigenetics -- The Gene Balance Hypothesis: Dosage Effects in Plants -- High-throughput RNA-Seq for Allelic or Locus-specific Expression Analysis in Arabidopsis Related Species, Hybrids, and Allotetraploids -- Inference of Allele-specific Expression from RNA-seq Data -- Screening for Imprinted Genes using High Resolution Melting Analysis of PCR Amplicons -- Analysis of Genomic Imprinting by Quantitative Allele-specific Expression by Pyrosequencing® -- Endosperm-specific Chromatin Profiling by Fluorescence-activated Nuclei Sorting and Chip-on-chip -- Imaging Sexual Reproduction in Arabidopsis using Fluorescent Markers -- Genome-wide Analysis of DNA Methylation in Arabidopsis using MeDIP-chip -- Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-stress Response in Montepulciano and Sangiovese Grape Cultivars -- Detecting Histone Modifications in Plants -- Quantitatively Profiling Genome-wide Patterns of Histone Modifications in Arabidopsis thaliana using ChIP-seq -- Analysis of Retrotransposon Activity in Plants -- Detecting Epigenetic Effects of Transposable Elements in Plants -- Detection and Investigation of Transitive Gene Silencing Effects in Plants.
520 _aMany fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols , leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape.  These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.       Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seeks to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aMolecular genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aMolecular Genetics.
700 1 _aSpillane, Charles.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMcKeown, Peter C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627037723
776 0 8 _iPrinted edition:
_z9781627037747
776 0 8 _iPrinted edition:
_z9781493962297
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1112
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-773-0
912 _aZDB-2-PRO
999 _c6628
_d6628