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001 978-1-60761-765-5
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005 20260610083649.0
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008 100825s2010 xxu| s |||| 0|eng d
020 _a9781607617655
_9978-1-60761-765-5
024 7 _a10.1007/978-1-60761-765-5
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Developmental Biology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Lars Hennig, Claudia Köhler.
250 _a1st ed. 2010.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2010.
300 _aXI, 448 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v655
505 0 _aGrowth Protocols for Model Plants in Developmental Biology -- Grafting as a Research Tool -- Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function -- The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level -- Inducible Gene Expression Systems for Plants -- Trichome Development in Arabidopsis -- Phenotyping the Development of Leaf Area in Arabidopsis thaliana -- Analyzing Shoot Apical Meristem Development -- Analyzing Floral Meristem Development -- Female Gametophytic Mutants: Diagnosis and Characterization -- Pollen Tube Development -- Analysis of Root Meristem Size Development -- Phenotypic Characterization of Photomorphogenic Responses During Plant Development -- Kinematic Analysis of Cell Division and Expansion -- Flowering Time Control -- mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis -- Immunolocalization of Proteins in Plants -- Detection of Small Non-coding RNAs -- Quantitative Real Time PCR in Plant Developmental Biology -- Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics -- Fluorescence-Activated Cell Sorting in Plant Developmental Biology -- Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling -- Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein–Protein Interaction in Plants -- The Split Luciferase Complementation Assay -- Co-immunoprecipitation and Protein Blots -- Probing Protein–Protein Interactions with FRET–FLIM -- Plant Chromatin Immunoprecipitation -- Immunocytological Analysis of Chromatin in Isolated Nuclei -- Bisulphite Sequencing of Plant Genomic DNA.
520 _aThe beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aDevelopmental biology.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aDevelopmental Biology and Stem Cells.
700 1 _aHennig, Lars.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aKöhler, Claudia.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781607617648
776 0 8 _iPrinted edition:
_z9781607617662
776 0 8 _iPrinted edition:
_z9781493957347
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v655
856 4 0 _uhttps://doi.org/10.1007/978-1-60761-765-5
912 _aZDB-2-PRO
999 _c6602
_d6602