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020 _a9781617793493
_9978-1-61779-349-3
024 7 _a10.1007/978-1-61779-349-3
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aChemical Genomics and Proteomics
_h[electronic resource] :
_bReviews and Protocols /
_cedited by Edward D. Zanders.
250 _a1st ed. 2012.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2012.
300 _aXI, 243 p. 71 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v800
505 0 _aOverview of Chemical Genomics and Proteomics -- Exploring Chemical Space: Recent Advances in Chemistry -- In Silico Design of Small Molecules -- Surface Plasmon Resonance for Proteomics -- Biomimetic Affinity Ligands for Immunoglobulins Based on the Multicomponent Ugi Reaction -- Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules -- Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids -- Fluorescence Labels in Kinases (FLiK): A High-Throughput Kinase Binding Assay for the Identification of DFG-Out Binding Ligands -- Electrochemical Aptamer Sensor for Small Molecule Assays -- Miniaturized, Microarray-Based Assays for Chemical Proteomic Studies of Protein Function -- Glycan Microarrays -- Resolving Bottlenecks for Recombinant Protein Expression in E. coli -- Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System -- Systems for the Cell-Free Synthesis of Proteins -- Protein Identification by MALDI-TOF Mass Spectrometry.
520 _aChemical genomics technology has been steadily improving, delivering new biological probes and drugs, and the explicit use of the term ‘chemical proteomics’ has increased with it, as proteins have always been at the heart of this technology.  In Chemical Genomics and Proteomics: Reviews and Protocols, experts in the field present updated reviews of the chemistry of small molecules and their interaction with protein targets as well as detailed protocols that cover different types of ligands, carbohydrates, and lipids.  For example, the generation of their protein targets and methods for measuring their interactions is covered.  Written in the highly successful Methods in Molecular Biology™ series format, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Thorough and up to date,  Chemical Genomics and Proteomics: Reviews and Protocols aims to provide inspiration to those who wish to use chemical genomics and proteomics in their work and develop this young field into full maturity through the incorporation of the new biological and chemical technologies beginning to emerge here.
650 0 _aBiochemistry.
650 0 _aProteins .
650 0 _aMedical genetics.
650 1 4 _aBiochemistry.
650 2 4 _aProtein Biochemistry.
650 2 4 _aMedical Genetics.
700 1 _aZanders, Edward D.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617793486
776 0 8 _iPrinted edition:
_z9781617793509
776 0 8 _iPrinted edition:
_z9781493962266
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v800
856 4 0 _uhttps://doi.org/10.1007/978-1-61779-349-3
912 _aZDB-2-PRO
999 _c6599
_d6599