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020 _a9781627039864
_9978-1-62703-986-4
024 7 _a10.1007/978-1-62703-986-4
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
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245 1 0 _aPlant-Pathogen Interactions
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Paul Birch, John T. Jones, Jorunn I.B. Bos.
250 _a2nd ed. 2014.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2014.
300 _aXIII, 306 p. 57 illus., 43 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1127
505 0 _aGalaxy as a Platform for Identifying Candidate Pathogen Effectors -- Bioinformatic analysis of expression data to identify effector candidates -- Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes -- On The Statistics Of Identifying Candidate Pathogen Effectors -- High-throughput imaging of plant immune responses -- In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats -- Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors -- Purification of fungal haustoria from infected plant tissue by flow cytometry -- Functional Characterisation of Nematode Effectors in Plants -- Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana -- Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids -- A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana -- Simple quantification of in planta fungal biomass -- Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum -- DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants -- A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector – Host Protein Complexes -- An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors -- Production of RXLR effector proteins for structural analysis by X-ray crystallography -- The Do’s and Don’ts of effectoromics -- Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice -- A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals.-Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.
520 _aPlant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify and characterize effectors and to study their impacts on host immunity and their roles in pathogen biology. Additional chapters focus on protocols to identify avirulence and resistance genes, investigate the roles of effector targets and other defence-associated proteins in plant immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Plant-Pathogen Interactions: Methods and Protocols, Second Edition seeks to aid scientists in the further study of plant immunity.
650 0 _aBotany.
650 0 _aPlant genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
700 1 _aBirch, Paul.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aJones, John T.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBos, Jorunn I.B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627039857
776 0 8 _iPrinted edition:
_z9781627039871
776 0 8 _iPrinted edition:
_z9781493963232
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1127
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-986-4
912 _aZDB-2-PRO
999 _c6582
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