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001 978-1-61737-957-4
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020 _a9781617379574
_9978-1-61737-957-4
024 7 _a10.1007/978-1-61737-957-4
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Chromosome Engineering
_h[electronic resource] :
_bMethods and Protocols /
_cedited by James A. Birchler.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXI, 340 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v701
505 0 _aRecent Advances in Plant Transformation -- Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics -- Homologous Recombination in Plants: An Anti-Review -- Chromosome Painting for Plant Biotechnology -- Plant B Chromosomes -- Telomere Truncation in Plants -- Engineered Plant Minichromosomes -- Method for Bxb1-Mediated Site-Specific Integration In planta -- Targeted Mutagenesis in Arabidopsis Using Zinc Finger Nucleases -- Vectors and Methods for Hairpin RNA and Artificial microRNA-Mediated Gene Silencing in Plants -- Recombinant Protein Expression in Nicotiana -- Chromosome Analysis and Sorting Using Flow Cytometry -- Super-Stretched Pachytene Chromosomes for Plant Molecular Cytogenetic Mapping -- Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System -- Development and Use of Oat-Maize Chromosome Additions and Radiation Hybrids -- Enhancer Trapping in Plants -- Chromatin Beacons: Global Sampling of Chromatin Physical Properties Using Chromatin Charting Lines -- Transposable Elements as Catalysts for Chromosome Rearrangements.
520 _aWith an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
650 0 _aBotany.
650 0 _aPlant genetics.
650 0 _aBiotechnology.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
650 2 4 _aChemical Bioengineering.
700 1 _aBirchler, James A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617379567
776 0 8 _iPrinted edition:
_z9781617379581
776 0 8 _iPrinted edition:
_z9781493957972
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v701
856 4 0 _uhttps://doi.org/10.1007/978-1-61737-957-4
912 _aZDB-2-PRO
999 _c6513
_d6513