000 04957nam a22004935i 4500
001 978-1-61737-988-8
003 DE-He213
005 20260610083648.0
007 cr nn 008mamaa
008 110104s2011 xxu| s |||| 0|eng d
020 _a9781617379888
_9978-1-61737-988-8
024 7 _a10.1007/978-1-61737-988-8
_2doi
050 4 _aQK
072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aPlant Embryo Culture
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Trevor A. Thorpe, Edward C. Yeung.
250 _a1st ed. 2011.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2011.
300 _aXII, 380 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v710
505 0 _aZygotic Embryo Culture: An Overview -- In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture -- Canola Zygotic Embryo Culture -- Immature Seeds and Embryos of Medicago truncatula Cultured In vitro -- In vitro Culture and Germination of Terrestrial Asian Orchid Seeds -- In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos -- Immature Embryo Rescue and Culture -- Chickpea Hybridization Using In vitro Techniques -- Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture -- Phaseolus Immature Embryo Rescue Technology -- Wide Crossing in Lentil through Embryo Rescue -- Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques -- Cryopreservation of Embryos: An Overview -- Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm -- Cryopreservation of Zygotic Embryo Axes and Somatic Embryos of European Chestnut -- Cryopreservation of Ilex Immature Zygotic Embryos -- The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview -- Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos -- Pine Somatic Embryogenesis Using Zygotic Embryos as Explants -- Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies -- Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview -- Genetic Transformation Using Maize Immature Zygotic Embryos -- Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue -- Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos.
520 _aA great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.
650 0 _aBotany.
650 0 _aCytology
_xTechnique.
650 1 4 _aPlant Science.
650 2 4 _aCytological Techniques.
700 1 _aThorpe, Trevor A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aYeung, Edward C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781617379871
776 0 8 _iPrinted edition:
_z9781617379895
776 0 8 _iPrinted edition:
_z9781493957897
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v710
856 4 0 _uhttps://doi.org/10.1007/978-1-61737-988-8
912 _aZDB-2-PRO
999 _c6508
_d6508