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020 _a9781592599486
_9978-1-59259-948-6
024 7 _a10.1007/978-1-59259-948-6
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
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245 1 0 _aChemical Genomics
_h[electronic resource] :
_bReviews and Protocols /
_cedited by Edward D. Zanders.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXII, 280 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v310
505 0 _aReviews -- to Chemical Genomics -- Chemistry for Chemical Genomics -- Computer-Aided Design of Small Molecules for Chemical Genomics -- Protocols -- Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography -- The Role and Application of In Silico Docking in Chemical Genomics Research -- Synthesis of Complex Carbohydrates and Glyconjugates -- High-Throughput Cloning for Proteomics Research -- Screening for the Expression of Soluble Recombinant Protein in Escherichia coli -- High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli -- The Wheat Germ Cell-Free Expression System -- Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System -- Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System -- Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening -- Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis -- Peptide and Small-Molecule Microarrays -- Peptide Mass Fingerprinting -- A Practical Protocol for Carbohydrate Microarrays -- Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors.
520 _aChemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aZanders, Edward D.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588293992
776 0 8 _iPrinted edition:
_z9781617375415
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v310
856 4 0 _uhttps://doi.org/10.1007/978-1-59259-948-6
912 _aZDB-2-PRO
999 _c6492
_d6492