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020 _a9781592599578
_9978-1-59259-957-8
024 7 _a10.1385/1592599575
_2doi
050 4 _aRS1-441
072 7 _aMQP
_2bicssc
072 7 _aMED072000
_2bisacsh
072 7 _aMQP
_2thema
082 0 4 _a615.1
_223
245 1 0 _aPharmacogenomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Federico Innocenti.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aX, 214 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v311
505 0 _aHistory and Overview -- Pharmacogenomics -- Functional Analysis of Gene Variation -- Transfection Assays With Allele-Specific Constructs -- Snapshot of the Allele-Specific Variation in Human Gene Expression -- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays -- HaploChIP -- Genotyping Techniques -- Aspects Influencing Genotyping Method Selection -- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping -- Pyrosequencing of Clinically Relevant Polymorphisms -- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation -- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry -- Fluorescence-Based Fragment Size Analysis -- Single-Nucleotide Polymorphism Genotyping in DNA Pools -- TaqMan Genotyping of Insertion/Deletion Polymorphisms -- Management of Pharmacogenomic Information -- PharmGKB -- Systems for the Management of Pharmacogenomic Information.
520 _aPharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.
650 0 _aPharmacy.
650 1 4 _aPharmacy.
700 1 _aInnocenti, Federico.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588294401
776 0 8 _iPrinted edition:
_z9781617375712
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v311
856 4 0 _uhttps://doi.org/10.1385/1592599575
912 _aZDB-2-PRO
999 _c6483
_d6483